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gcf samples  (NanoView Biosciences)

 
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    Structured Review

    NanoView Biosciences gcf samples
    A) Schematic <t>of</t> <t>NanoView</t> Biosciences detection system. Antibodies to EV surface antigens are attached to a signal enhancing surface. EVs are then incubated on the spots and unbound sample is washed free. Bound EVs can then be detected by interferometrics or by fluorescent labeling. B) <t>GCF</t> was collected from the buccal side of the left upper central incisor by a single Periopaper dip following the method described in detail by our group previously (2) and following a procedure approved by the University of Florida Internal Review Board (IRB201600476). The GCF was analyzed using the ExoView Platform on an anti-CD63 spot as described previously (25). GCF was eluted with 100 μl PBS. Twenty μl of the sample was diluted 1:1 with PBS then introduced into the ExoView apparatus. Samples were incubated for 12 H, washed and labeled for 2 H with antibody, washed again and nanoparticles were detected by either light scatter or fluorescence. Round circles indicate particles detected by light scatter. Bright particles were those labeled with anti-CD63. Most of the anti-CD63 labeled EVs were not detected by the interferometric method indicating the CD63 labeled particles are mostly smaller than about 60 nm in diameter, the detection limit of the interferometric technique.
    Gcf Samples, supplied by NanoView Biosciences, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/gcf+samples/gcf+samples/pmc06512852-165-4-8
    Average 90 stars, based on 1 article reviews
    gcf samples - by Bioz Stars, 2026-09
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    Images

    1) Product Images from "Extracellular vesicle identification in tooth movement models"

    Article Title: Extracellular vesicle identification in tooth movement models

    Journal: Orthodontics & craniofacial research

    doi: 10.1111/ocr.12287

    A) Schematic of NanoView Biosciences detection system. Antibodies to EV surface antigens are attached to a signal enhancing surface. EVs are then incubated on the spots and unbound sample is washed free. Bound EVs can then be detected by interferometrics or by fluorescent labeling. B) GCF was collected from the buccal side of the left upper central incisor by a single Periopaper dip following the method described in detail by our group previously (2) and following a procedure approved by the University of Florida Internal Review Board (IRB201600476). The GCF was analyzed using the ExoView Platform on an anti-CD63 spot as described previously (25). GCF was eluted with 100 μl PBS. Twenty μl of the sample was diluted 1:1 with PBS then introduced into the ExoView apparatus. Samples were incubated for 12 H, washed and labeled for 2 H with antibody, washed again and nanoparticles were detected by either light scatter or fluorescence. Round circles indicate particles detected by light scatter. Bright particles were those labeled with anti-CD63. Most of the anti-CD63 labeled EVs were not detected by the interferometric method indicating the CD63 labeled particles are mostly smaller than about 60 nm in diameter, the detection limit of the interferometric technique.
    Figure Legend Snippet: A) Schematic of NanoView Biosciences detection system. Antibodies to EV surface antigens are attached to a signal enhancing surface. EVs are then incubated on the spots and unbound sample is washed free. Bound EVs can then be detected by interferometrics or by fluorescent labeling. B) GCF was collected from the buccal side of the left upper central incisor by a single Periopaper dip following the method described in detail by our group previously (2) and following a procedure approved by the University of Florida Internal Review Board (IRB201600476). The GCF was analyzed using the ExoView Platform on an anti-CD63 spot as described previously (25). GCF was eluted with 100 μl PBS. Twenty μl of the sample was diluted 1:1 with PBS then introduced into the ExoView apparatus. Samples were incubated for 12 H, washed and labeled for 2 H with antibody, washed again and nanoparticles were detected by either light scatter or fluorescence. Round circles indicate particles detected by light scatter. Bright particles were those labeled with anti-CD63. Most of the anti-CD63 labeled EVs were not detected by the interferometric method indicating the CD63 labeled particles are mostly smaller than about 60 nm in diameter, the detection limit of the interferometric technique.

    Techniques Used: Incubation, Labeling, Fluorescence

    Related Articles

    Incubation:

    Article Title: Extracellular vesicle identification in tooth movement models
    Article Snippet: detection of unlabeled EVs through interferometric data, it is also possible to label the bound EVs using antibodies and detect them by fluorescence. .. In preliminary runs of GCF samples (courtesy of NanoView Biosciences, Boston MA) in an ExoView platform, both CD9 and CD63 containing EVs were detected in GCF ( ). .. The EVs detected by fluorescence were mostly not detected by light scatter.

    Labeling:

    Article Title: Extracellular vesicle identification in tooth movement models
    Article Snippet: detection of unlabeled EVs through interferometric data, it is also possible to label the bound EVs using antibodies and detect them by fluorescence. .. In preliminary runs of GCF samples (courtesy of NanoView Biosciences, Boston MA) in an ExoView platform, both CD9 and CD63 containing EVs were detected in GCF ( ). .. The EVs detected by fluorescence were mostly not detected by light scatter.

    Fluorescence:

    Article Title: Extracellular vesicle identification in tooth movement models
    Article Snippet: detection of unlabeled EVs through interferometric data, it is also possible to label the bound EVs using antibodies and detect them by fluorescence. .. In preliminary runs of GCF samples (courtesy of NanoView Biosciences, Boston MA) in an ExoView platform, both CD9 and CD63 containing EVs were detected in GCF ( ). .. The EVs detected by fluorescence were mostly not detected by light scatter.



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    A) Schematic <t>of</t> <t>NanoView</t> Biosciences detection system. Antibodies to EV surface antigens are attached to a signal enhancing surface. EVs are then incubated on the spots and unbound sample is washed free. Bound EVs can then be detected by interferometrics or by fluorescent labeling. B) <t>GCF</t> was collected from the buccal side of the left upper central incisor by a single Periopaper dip following the method described in detail by our group previously (2) and following a procedure approved by the University of Florida Internal Review Board (IRB201600476). The GCF was analyzed using the ExoView Platform on an anti-CD63 spot as described previously (25). GCF was eluted with 100 μl PBS. Twenty μl of the sample was diluted 1:1 with PBS then introduced into the ExoView apparatus. Samples were incubated for 12 H, washed and labeled for 2 H with antibody, washed again and nanoparticles were detected by either light scatter or fluorescence. Round circles indicate particles detected by light scatter. Bright particles were those labeled with anti-CD63. Most of the anti-CD63 labeled EVs were not detected by the interferometric method indicating the CD63 labeled particles are mostly smaller than about 60 nm in diameter, the detection limit of the interferometric technique.
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    A) Schematic <t>of</t> <t>NanoView</t> Biosciences detection system. Antibodies to EV surface antigens are attached to a signal enhancing surface. EVs are then incubated on the spots and unbound sample is washed free. Bound EVs can then be detected by interferometrics or by fluorescent labeling. B) <t>GCF</t> was collected from the buccal side of the left upper central incisor by a single Periopaper dip following the method described in detail by our group previously (2) and following a procedure approved by the University of Florida Internal Review Board (IRB201600476). The GCF was analyzed using the ExoView Platform on an anti-CD63 spot as described previously (25). GCF was eluted with 100 μl PBS. Twenty μl of the sample was diluted 1:1 with PBS then introduced into the ExoView apparatus. Samples were incubated for 12 H, washed and labeled for 2 H with antibody, washed again and nanoparticles were detected by either light scatter or fluorescence. Round circles indicate particles detected by light scatter. Bright particles were those labeled with anti-CD63. Most of the anti-CD63 labeled EVs were not detected by the interferometric method indicating the CD63 labeled particles are mostly smaller than about 60 nm in diameter, the detection limit of the interferometric technique.
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    A) Schematic <t>of</t> <t>NanoView</t> Biosciences detection system. Antibodies to EV surface antigens are attached to a signal enhancing surface. EVs are then incubated on the spots and unbound sample is washed free. Bound EVs can then be detected by interferometrics or by fluorescent labeling. B) <t>GCF</t> was collected from the buccal side of the left upper central incisor by a single Periopaper dip following the method described in detail by our group previously (2) and following a procedure approved by the University of Florida Internal Review Board (IRB201600476). The GCF was analyzed using the ExoView Platform on an anti-CD63 spot as described previously (25). GCF was eluted with 100 μl PBS. Twenty μl of the sample was diluted 1:1 with PBS then introduced into the ExoView apparatus. Samples were incubated for 12 H, washed and labeled for 2 H with antibody, washed again and nanoparticles were detected by either light scatter or fluorescence. Round circles indicate particles detected by light scatter. Bright particles were those labeled with anti-CD63. Most of the anti-CD63 labeled EVs were not detected by the interferometric method indicating the CD63 labeled particles are mostly smaller than about 60 nm in diameter, the detection limit of the interferometric technique.
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    Image Search Results


    A) Schematic of NanoView Biosciences detection system. Antibodies to EV surface antigens are attached to a signal enhancing surface. EVs are then incubated on the spots and unbound sample is washed free. Bound EVs can then be detected by interferometrics or by fluorescent labeling. B) GCF was collected from the buccal side of the left upper central incisor by a single Periopaper dip following the method described in detail by our group previously (2) and following a procedure approved by the University of Florida Internal Review Board (IRB201600476). The GCF was analyzed using the ExoView Platform on an anti-CD63 spot as described previously (25). GCF was eluted with 100 μl PBS. Twenty μl of the sample was diluted 1:1 with PBS then introduced into the ExoView apparatus. Samples were incubated for 12 H, washed and labeled for 2 H with antibody, washed again and nanoparticles were detected by either light scatter or fluorescence. Round circles indicate particles detected by light scatter. Bright particles were those labeled with anti-CD63. Most of the anti-CD63 labeled EVs were not detected by the interferometric method indicating the CD63 labeled particles are mostly smaller than about 60 nm in diameter, the detection limit of the interferometric technique.

    Journal: Orthodontics & craniofacial research

    Article Title: Extracellular vesicle identification in tooth movement models

    doi: 10.1111/ocr.12287

    Figure Lengend Snippet: A) Schematic of NanoView Biosciences detection system. Antibodies to EV surface antigens are attached to a signal enhancing surface. EVs are then incubated on the spots and unbound sample is washed free. Bound EVs can then be detected by interferometrics or by fluorescent labeling. B) GCF was collected from the buccal side of the left upper central incisor by a single Periopaper dip following the method described in detail by our group previously (2) and following a procedure approved by the University of Florida Internal Review Board (IRB201600476). The GCF was analyzed using the ExoView Platform on an anti-CD63 spot as described previously (25). GCF was eluted with 100 μl PBS. Twenty μl of the sample was diluted 1:1 with PBS then introduced into the ExoView apparatus. Samples were incubated for 12 H, washed and labeled for 2 H with antibody, washed again and nanoparticles were detected by either light scatter or fluorescence. Round circles indicate particles detected by light scatter. Bright particles were those labeled with anti-CD63. Most of the anti-CD63 labeled EVs were not detected by the interferometric method indicating the CD63 labeled particles are mostly smaller than about 60 nm in diameter, the detection limit of the interferometric technique.

    Article Snippet: In preliminary runs of GCF samples (courtesy of NanoView Biosciences, Boston MA) in an ExoView platform, both CD9 and CD63 containing EVs were detected in GCF ( ).

    Techniques: Incubation, Labeling, Fluorescence